4-Dimensional Cellular Smokestacks

Year: 
2022
Ranking: 
Entrant
Artist: 
Ryan Lach, graduate student
Department: 
Molecular, Cellular, and Developmental Biology

Description

Proteins that control a stem cell's fate form large, dynamic droplets in the cytoplasm. When droplets are tracked through time and space simultaneously, 'smokestacks' allow the viewer to follow the evolution of each's structure in a single image.

A 3-Dimensional volumetric projection of a single, live cell's fluorescently-tagged Axin droplets at 168X magnification, color-coded by the time at which the image was taken, from green (earlier) to yellow (later). Image was taken on Nikon W1 SoRa super-resolution, spinning-disk confocal microscope. The image's timecourse spans only 60 seconds, highlighting the constant rearrangement of droplets in space and time.

CSEPMOXICNSISchuller LabUCSB